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以籼粳交“密阳23/吉冷1号”的F2:3 代200个家系为作图群体,构建分子连锁图谱,并进行了F3 代家系的生长早期耐冷性鉴定和QTL分析。结果表明,幼苗期耐冷性、分蘖期耐冷性和低温下幼苗生长能力等生长早期耐冷性在F3 代表现为近似正态的连续分布,是由多基因控制的数量性状。在第1、5、9 染色体上分别检测到与幼苗期耐冷性相关的QTL各1个,其中qCTS1 对表型变异的解释率最大,达15.5%;在第2、3、7、9、11 染色体上分别检测到与分蘖期耐冷性相关的QTL各1个,而每个QTL对表型变异的解释率均较低;在第1、2、11、12染色体上分别检测到与低温下幼苗生长能力相关的QTL各1个,其中qGAS2 和qGAS12 对表型变异的解释率分别为26.6%和42.9%,是主效基因。
A F2 population of F2: 3 generations from “Mianyang 23 / Ji Leng 1” indica and japonica hybrids was used as a mapping population to construct molecular linkage map, and the cold tolerance identification and QTL analysis of the F3 generation lines were carried out. The results showed that the early cold tolerance at seedling stage such as cold tolerance, cold tolerance at tillering stage and seedling growth ability at low temperature showed a nearly normal distribution continuously in F3, which was a quantitative trait controlled by polygenes. One QTL related to cold tolerance was detected on chromosomes 1, 5 and 9, respectively, of which qCTS1 had the highest rate of phenotypic variation (15.5%). In the second, third, seventh, ninth and eleventh Chromosomes were detected with QTLs related to cold tolerance at tillering stage each one, and each QTL explained the phenotypic variation are low; respectively detected on chromosomes 1, 2, 11, and 12 and low temperature seedlings There was one QTL related to growth ability, of which qGAS2 and qGAS12 explained 26.6% and 42.9% of the phenotypic variation, respectively.