Adsorption properties of L-histidine on active carbon were studied in the paper, which are affected by the main parameters, such as the quantity percent of active carbon, pH value of the solution, the time of adsorption equilibrium and adsorption temperature. The results indicate that adsorption equilibrium time of L-his on active carbon is about 80 minutes. With the increasing of the quantity percent of active carbon, the adsorbance of L-his decreases sharply, and increases lighter after that. When the quantity percent of active carbon is 10%, the adsorbance reaches the minimum. pH value of solution and extraction temperature have great affection on the adsorption. When the pH value is higher or lower than the pI of L-his, the adsorbance is small, even zero. It is proven that the experimental equilibrium data which are obtained under the conditions of 80℃and pH=1.0, are fitted with the Freundlich equation: q=2.5914c0.8097. The results can provide certain references in L-his adsorption process of industrial operation. Adsorption properties of L-histidine on active carbon were studied in the paper, which are affected by the main parameters, such as the quantity percent of active carbon, pH value of the solution, the time of adsorption equilibrium and adsorption temperature. that the adsorption equilibrium time of L-his on active carbon is about 80 minutes. With the increasing of the quantity percent of active carbon, the adsorbance of L-his decreases sharply, and increases pounds after that. When the quantity percent of active carbon is 10%, the adsorbance reaches the minimum. PH value of solution and extraction temperature have great affection on the adsorption. When the pH value is higher or lower than the pI of L-his, the adsorbance is small, even zero. It is proven that the experimental equilibrium data which are obtained under the conditions of 80 ° C and pH = 1.0, are fitted with the Freundlich equation: q = 2.5914c0.8097. The results can provide certain references in L-his adsorpti on process of industrial operation.