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目的 观察海水温度变化对腹腔海水浸泡伤大鼠存活的影响。方法 SD大鼠 2 4只 ,随机均分为 32℃海水浸泡组 (A组 )、2 2℃海水浸泡组 (B组 )及 1 2℃海水浸泡组 (C组 ) ,观察每一组腹腔海水浸泡前后平均动脉压 (MAP)、心率 (HR)、乳酸 (LA)、乳酸脱氢酶 (LDH)、谷丙转氨酶 (ALT)、血淀粉酶 (AMY)、尿素氮(BUN)及肌酸激酶同工酶 (CKMB)的变化。另取大鼠 30只 ,随机均分为 32℃海水浸泡组 (D组 )、2 2℃海水浸泡组 (E组 )及 1 2℃海水浸泡组 (F组 ) ,用以观察大鼠持续腹腔海水浸泡后的存活时间。结果 (1 )F组平均存活时间仅为 6 5分钟 ,显著少于D组 (4 81分钟 )及E组 (2 1 7分钟 ) (P <0 .0 1 ) ;(2 )浸泡 30分钟及浸泡 3小时后B、C组MAP、HR与浸泡前及与A组比较均发生显著下降 (P <0 .0 5 ) ,而C组MAP、HR显著低于B组 (P<0 .0 5 ) ;(3)浸泡后 3小时 ,C组血浆LA、LDH、ALT、AMY、BUN及CKMB水平均显著高于A、B组(P <0 .0 1 )。结论 低温海水浸泡大鼠腹腔开放伤可引起大鼠机体血流动力学迅速发生严重紊乱、代谢恶化及内脏功能损伤 ,导致大鼠快速死亡
Objective To observe the effects of seawater temperature changes on the survival of rats with celiac seawater immersion injury. Methods Twenty-four Sprague-Dawley rats were randomly divided into three groups: seawater immersion (group A) at 32 ℃, seawater immersion at 22 ℃ (group B) and seawater immersion (group C) at 12 ℃. The changes of MAP, HR, LDH, ALT, AMY, BUN and creatine kinase before and after soaking were observed. Isoenzyme (CKMB) changes. Thirty rats were randomly divided into three groups: seawater immersion group (group D), seawater immersion group (group E) at 20 ℃ and seawater immersion group (group F) at 12 ℃, respectively. Survival time after seawater immersion. Results (1) The average survival time in group F was only 65 minutes, significantly less than those in group D (4 81 minutes) and group E (217 days) (P <0.01); (2) After soaking for 3 hours, MAP, HR in B and C groups decreased significantly compared with that in A group (P <0.05), while MAP and HR in C group were significantly lower than those in B group (P <0.05) ); (3) Three hours after soaking, the levels of LA, LDH, ALT, AMY, BUN and CKMB in group C were significantly higher than those in group A and B (P <0.01). CONCLUSION: Open abdominal injury caused by low temperature seawater immersion can cause serious hemodynamics disorder, metabolic deterioration and visceral dysfunction in rats rapidly leading to rapid death in rats