论文部分内容阅读
为研究防污剂Irgarol 1051对微藻生长及生理生化特性的影响,以三角褐指藻(Phaeodactylum tricornutum)为材料,分析了Irgarol 1051对P.tricornutum生长、叶绿素a含量、可溶性蛋白质含量、超氧化物歧化酶(Superoxide dismutase,SOD)活力及丙二醛(Malondialdehyde,MDA)含量等的影响。结果表明:Irgarol 1051可抑制P.tricornutum的生长,并诱导其产生耐受性;P.tricornutum中叶绿素a、可溶性蛋白质含量及SOD酶活力等均随Irgarol 1051质量浓度的增加而升高,以维持其生长和生理状态;随着Irgarol 1051质量浓度的升高,藻细胞中MDA含量显著增加,表明细胞中活性氧自由基(Reactive oxygen species,ROS)过量积累,这将破坏藻细胞的膜结构与功能。研究结果将为探讨Irgarol1051对微藻的效应机制及其环境风险评价提供数据资料和科学依据。
In order to study the effect of anti-fouling agent Irgarol 1051 on the growth and physiological and biochemical characteristics of microalgae, Phaeodactylum tricornutum was used to study the effects of Irgarol 1051 on the growth of P.tricornutum, the content of chlorophyll a, soluble protein, The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) The results showed that Irgarol 1051 inhibited the growth of P.tricornutum and induced its tolerance. Chlorophyll a, soluble protein content and SOD activity of P.tricornutum increased with the increase of Irgarol 1051 concentration in order to maintain Its growth and physiological status. With the increase of the concentration of Irgarol 1051, the content of MDA in algal cells increased significantly, indicating that excessive accumulation of reactive oxygen species (ROS) in the cells would destroy the membrane structure of algae cells and Features. The results will provide data and scientific basis for exploring the mechanism of Irgarol 1051 on the microalgae and its environmental risk assessment.