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采用浸浴法,研究低分子质量壳聚糖(LMWC)对镉胁迫引起河南华溪蟹(Sinopotamon henanense)肝胰腺组织中镉积累情况、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量、谷胱甘肽硫转移酶(GST)活性和还原型谷胱甘肽(GSH)含量的影响,旨在评价LMWC的保护作用。设置镉处理组(29、58、87mg/L)、LMWC组(20、40、80mg/L)、LMWC组(20、40、80mg/L)和镉(58mg/L)联合处理组,对照组;处理时间为96h。结果显示:与对照组相比,LMWC单独作用下,SOD活性和MDA含量变化不显著,不会引起肝胰腺组织脂质过氧化。但58mg/L镉单独作用下,SOD活性显著降低,MDA含量显著增加,降低了机体抗氧化能力。镉积累量随着镉浓度升高逐渐增加,加入LMWC后镉积累量显著降低,且具有质量浓度-效应关系。GST活性和GSH含量随着镉质量浓度升高逐渐降低,而加入LMWC后其水平比镉染毒组(58mg/L)显著升高。LMWC对镉胁迫引起的肝胰腺组织氧化损伤可以起到良好的保护作用。
The immersion method was used to study the effects of low molecular weight chitosan (LMWC) on the cadmium accumulation, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in hepatopancreas of Sinopotamon henanense, ) Content, glutathione S-transferase (GST) activity and reduced glutathione (GSH) content in order to evaluate the protective effect of LMWC. The rats in control group (29, 58, 87mg / L), LMWC group (20,40,80mg / L), LMWC group (20,40,80mg / L) and cadmium ; Processing time is 96h. The results showed that, compared with the control group, under the action of LMWC alone, SOD activity and MDA content did not change significantly, and did not cause lipid peroxidation in hepatopancreas tissue. However, under the action of 58mg / L cadmium alone, the activity of SOD was significantly decreased and MDA content was significantly increased, which reduced the antioxidant capacity of the body. The accumulation of cadmium increased with the increase of cadmium concentration. The addition of LMWC significantly reduced the accumulation of cadmium, and had a mass concentration - effect relationship. GST activity and GSH content gradually decreased with the increase of cadmium concentration, but the level of GST activity was significantly higher than that of cadmium exposure group (58mg / L) after adding LMWC. LMWC can protect hepatopancreas from oxidative damage caused by cadmium stress.