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淋巴结HE的制片是常规病理技术工作中的重点与难点。由于淋巴结外表被一层厚厚的包膜所包裹,内部又由细胞致密的皮质、髓质所构成,导致制片过程中经常出现组织固定不充分、脱水不彻底、切片较厚、灰染、镜下细胞重叠等现象,给病理诊断造成困扰,甚至被定为“疑难病例”[1]。本文着力于探索淋巴结HE制片过程中的影响因素,交流制片的方法与技巧,提高淋巴结HE切片的优良率。
The production of lymph node HE is the focus and difficulty of routine pathological techniques. Due to the appearance of the lymph nodes is covered by a thick layer of envelopes, the interior by the dense cell cortex, medulla constitute, resulting in the filming process often appear inadequate tissue fixation, dehydration is not complete, thick slices, gray, Microscopic overlap of cells and other phenomena that cause trouble to the pathological diagnosis, or even as “difficult cases ” [1]. This article focuses on exploring the influencing factors in the process of lymph node HE preparation, the methods and techniques of exchanging the films and improving the excellent rate of HE slice in lymph nodes.