目的　观察小型猪冠状动脉置入支架后p2 7kip表达变化及含 10 0 μg雷帕霉素可降解涂层支架释放的雷帕霉素对p2 7kip表达的影响。方法　球囊 血管以 1 3∶1比例置入过大的裸支架 (n =14 )、单高分子可降解多聚羟基丙酸乙酸 (PLGA)涂层支架 (n =16 )或雷帕霉素支架 (n =16 )并形成冠状动脉损伤模型 ,术后第 1、2、4和 12周时间段处死部分猪。测定 12周时 3组支架血管段的内膜厚度和面积。免疫组化法测定支架置入后 4个时段冠状动脉的p2 7kip表达水平及动态变化。结果　在 12周的随访组 ,裸支架、PLGA支架和雷帕霉素支架的新生内膜平均厚度分别为 (0 38± 0 2 0 )mm、(0 96± 0 5 8)mm和 (0 14± 0 12 )mm(P =0 0 0 4 ) ,3组的新生内膜面积分别为 (3 38± 0 31)mm2 、(6 4 6±2 0 1)mm2 和 (2 0 7± 0 82 )mm2 (P =0 0 0 0 )。置入裸支架 1周后 ,冠状动脉p2 7kip表达处于低水平状态 ,但在第 4周达到最高水平 ,在第 12周时 ,其表达水平恢复至第 1周时水平 ;雷帕霉素支架使整个随访期的p2 7kip表达水平无明显变化 ,均处于高水平表达状态。结论　含 10 0 μg雷帕霉素支架在 12周内有抑制小型猪冠状动脉内膜增殖的明显疗效。裸支架置入后 12周内 ,p2 7kip表达水平在第 4周最高 ;涂层支架释放的? Objective To investigate the expression of p2 7 kip after implantation of coronary stents in miniature pigs and the effect of rapamycin on the expression of p2 7 kip with 100 μg rapamycin-degradable stent. Methods Balloon vessels were placed in an oversized bare stent (n = 14) in a 1: 3: 1 ratio. Single macromolecule biodegradable PLGA coated stents (n = 16) or rapamycin (N = 16) and formed a model of coronary artery injury. Some pigs were sacrificed at the 1st, 2nd, 4th, and 12th week after operation. The thickness and area of ​​intima of the vascular segments of the three groups were measured at 12 weeks. Immunohistochemistry was used to detect the expression of p2 7kip in coronary artery at 4 time points after stent implantation. Results The mean neointimal thickness of the bare stent, PLGA stent and rapamycin stent were (0 38 ± 0 2 0) mm, (0 96 ± 0 58) mm and (0 14 ± 0 12) mm (P = 0 0 0 4). The neointimal area in the 3 groups was (3 38 ± 0 31) mm 2, (6 4 6 ± 2 0 1) mm 2 and (2 0 7 ± 0 82 ) mm2 (P = 0 0 0 0). After 1 week of implantation, the expression of p2 7kip in coronary artery was at a low level but reached the highest level at the 4th week, and returned to the level at 1 week after the 12th week. The p2 7kip expression level did not change significantly during the whole follow-up period, all of which were in high level expression. Conclusion The scaffold containing 10 0 μg rapamycin can inhibit the proliferation of coronary intima of miniature pigs within 12 weeks. Within 12 weeks after bare stent implantation, the expression level of p2 7kip was the highest in the 4th week;