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以豫椒系列品种父母本和杂交种为材料,采用液氮-SDS法提取辣椒基因组DNA,使用Bio-RAD-Mycy-cler型PCR仪和Bio-RAD1000凝胶扫描仪,研究PCR反应的主要影响因子,建立适合辣椒RAPD分析的PCR反应体系,从40个10bp的随机引物中筛选出1个能鉴定豫椒968杂种纯度的引物S149。
The pepper genomic DNA was extracted by liquid nitrogen-SDS method using the parents and hybrids of the varieties of pepper varieties. The main effects of the PCR reaction were studied using a Bio-RAD-Mycy-cler PCR instrument and a Bio-RAD1000 gel scanner Factor to establish a PCR reaction system suitable for RAPD analysis of pepper. A primer S149 that can identify the purity of the 968 hybrid pepper was screened from 40 10bp random primers.