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目的探讨苹果多酚体外对乳腺癌细胞MDA-MB-231增殖、迁移的影响及可能的作用机制。方法对数生长期乳腺癌细胞用0、50、100、200、400和800μg/m L苹果多酚处理24、48和72 h,台盼蓝染色测细胞活力;CCK8试剂盒测细胞生长和增殖;划痕实验观察细胞迁移能力的变化;Western blot检测蛋白质表达水平。结果0~800μg/m L范围内,苹果多酚可浓度依赖性地抑制细胞活力和细胞增殖,下调泛素样含PHD和环指域1(ubiquitinlike with PHD and ring finger domain 1,UHRF1)和基质金属蛋白酶-2(matrix metalloproteinases-2,MMP2)的表达,以及UHRF1的调控靶分子DNA甲基转移酶3a(DNA methyltransferases 3a,DNMT3a)和3b(DNMT3b)的表达水平;而苹果多酚抑制细胞迁移的作用在400~800μg/m L浓度范围则比较显著。结论苹果多酚通过下调UHRF1和MMP2的表达,抑制乳腺癌细胞MDA-MB-231的活力、增殖和迁移。
Objective To investigate the effect of apple polyphenols on the proliferation and migration of breast cancer cell MDA-MB-231 in vitro and its possible mechanism. Methods The logarithmic growth phase breast cancer cells were treated with apple polyphenols at 0, 50, 100, 200, 400 and 800 μg / mL for 24, 48 and 72 h. Cell viability was measured by trypan blue staining. Cell growth and proliferation were measured by CCK8 kit Scratch experiment was used to observe the changes of cell migration ability. Western blot was used to detect the protein expression level. Results Apple polyphenols inhibited cell viability and cell proliferation in a dose - dependent manner in the range of 0 ~ 800 μg / m L, down - regulated ubiquitin - like PHD and ubiquitin - like with PHD and ring finger domain 1 (UHRF1) and matrix The expression of matrix metalloproteinases-2 (MMP2), as well as the expression of DNA methyltransferases 3a (DNMT3a) and 3b (DNMT3b) regulated by UHRF1, while apple polyphenols inhibited cell migration The role of 400 ~ 800μg / m L concentration range is more significant. Conclusion Apple polyphenols can inhibit the activity, proliferation and migration of breast cancer cell MDA-MB-231 by down-regulating the expression of UHRF1 and MMP2.