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目的研究人骨髓细胞中自然抑制细胞的抗肿瘤效应。方法分离并培养人骨髓中不同的细胞亚群，将不同的骨髓细胞亚群或其培养上清加入HL-6O或Kato-3肿瘤细胞培养体系中，检测肿瘤细胞的增殖反应，并检测电离辐射及蛋白质合成阻断剂对骨髓自然抑制活性的影响。结果具有自然抑制活性的骨髓细胞主要为CD34+CD33-细胞，其不但可以抑制丝裂素原刺激的人外周血单个核细胞的增殖，而且可以抑制肿瘤细胞株的增殖。自然抑制细胞发挥其自然抑制活性不需要细胞接触，但是蛋白质的合成则是这一过程所必需的，因为嘌呤霉素阻断自然抑制细胞发挥其自然抑制活性。此外，我们的结果表明，自然抑制细胞可分泌一种可溶性因子，IL－3和GM－GSF可以促进这一可溶性因子的产生。结论人骨髓细胞中自然抑制细胞具有显著的抗肿瘤效应。
Objective To study the anti-tumor effects of natural suppressor cells in human bone marrow cells. METHODS: Different cell subpopulations in human bone marrow were isolated and cultured, and different subpopulations of bone marrow cells or their culture supernatants were added to HL-6O or Kato-3 tumor cell culture system to examine the proliferative response of tumor cells and detect ionizing radiation. And the effect of protein synthesis blockers on the natural inhibitory activity of bone marrow. As a result, bone marrow cells having natural inhibitory activity were mainly CD34+CD33-cells, which not only inhibited the proliferation of human peripheral blood mononuclear cells stimulated by mitogen, but also inhibited the proliferation of tumor cell lines. Naturally inhibiting cells to exert their natural inhibitory activity does not require cell contact, but protein synthesis is necessary for this process because puromycin blocks naturally inhibiting cells to exert their natural inhibitory activity. In addition, our results indicate that natural inhibitory cells secrete a soluble factor, and IL-3 and GM-GSF can promote the production of this soluble factor. Conclusion The natural inhibitory cells in human bone marrow cells have a significant anti-tumor effect.