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目的探讨新疆南部地区维吾尔族妇女宫颈癌组织中人乳头状瘤病毒16型(human papillomavirus 16,HPV-16)上游调控区(upstream regulatory region,URR)的变异及其与新疆维吾尔族妇女宫颈癌发生的关系。方法从19份中国新疆南部地区维吾尔族妇女宫颈癌活检组织标本中提取DNA,以此DNA为模板,PCR扩增HPV-16 URR DNA片段,PCR产物直接测序或克隆后测序,分析新疆维吾尔族妇女宫颈癌组织HPV-16 URR DNA多态性。结果PCR检测结果显示宫颈癌组织中HPV-16 URR阳性率为89．47％(17/19)；测序和序列分析表明,与HPV-16原型比较,HPV-16 URR核苷酸多处发生变异,URR在核苷酸水平上形成11种突变模式(XJU-1～XJU-11),XJU-1和XJU-4突变模式各占23．53％(4/17),其余各突变模式均占5．88％(1/17),各模式与HPV-16 URR原型比较,同源性在98．50％～99．68％之间。在各位点的突变中,7192位(G→T)、7520位(G→A)的突变(100％,17/17)在新疆地区趋于恒定,该两处突变在亚裔美洲型(AA)中普遍存在,与病毒感染及宫颈癌发生相关；7729位(A→C)、7843位(A→G)、7792位(C→T)的突变可显著提高启动子的转录活性；其余突变尚未见报道,部分突变为新疆地区分离的HPV-16 URR所特有。结论中国新疆南部HPV-16 URR基因发生变异,HPV-16 URR的突变与HPV-16的系统发生以及新疆维吾尔族妇女高发宫颈癌存在一定关系。
Objective To investigate the variation of upstream regulatory region (URR) of human papillomavirus 16 (HPV-16) in Uigur women from southern Xinjiang and their association with cervical cancer in Uighur women in Xinjiang Relationship. Methods DNA was extracted from 19 samples of uterine cervix cancer biopsies from Uygur women in southern Xinjiang, China. The DNA was used as a template to amplify HPV-16 URR DNA fragments. The PCR products were sequenced directly or sequenced. The prevalence of Uygur women Cervical Cancer HPV-16 URR DNA Polymorphism. Results The positive rate of HPV-16 URR in cervical cancer tissues was 89.47% (17/19) by PCR. Sequencing and sequence analysis showed that HPV-16 URR nucleotides mutated in many places compared with HPV-16 prototype , URR formed 11 kinds of mutation patterns (XJU-1 ~ XJU-11) at the nucleotide level, 23.53% (4/17) in XJU-1 and XJU-4, and the other mutation patterns accounted for 5.88% (1/17). The patterns of HPV-16 URR prototypes were compared with each other and the homologies were between 98.50% and 99.68%. Among the mutations at each locus, mutations at 7192 (G → T) and 7520 (G → A) (100%, 17/17) tended to be constant in Xinjiang, ), Which was associated with viral infection and cervical cancer. The mutations at positions 7729 (A → C), 7843 (A → G) and 7792 (C → T) significantly increased the transcriptional activity of the promoter. The remaining mutations It has not been reported that some mutations are unique to HPV-16 URR isolated in Xinjiang. Conclusions The HPV-16 URR gene is mutated in the southern part of Xinjiang, China. The mutation of HPV-16 URR is related to the phylogeny of HPV-16 and the high incidence of cervical cancer in Uighur women in Xinjiang.