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为探讨甘蔗属内不同种之间的遗传多样性,利用SSR标记与毛细管电泳技术,对来自甘蔗属3个不同种的12个材料19对引物进行检测,共检测到229个DNA多态性条带,19对引物扩增的DNA条带范围集中在100～260bp之间。12个甘蔗材料的Jaccard遗传相似度,最小0.09,最大0.65,平均为0.26。通过遗传相似性系数分析,UPGMA聚类图内12个甘蔗材料可分为两个群,三个割手密种材料分为一个亚群,甘蔗栽培品种与甘蔗热带种合为一个亚群。结果表明:热带种比割手密种具有和甘蔗栽培品种更亲近的遗传关系;SSR分子标记与毛细管技术结合,相比别的分子标记技术或电泳技术,具有更准确、简便、自动化等优点。
In order to explore the genetic diversity among different species in Saccharum, 19 primer pairs of 12 materials from 3 different species of Saccharum were detected by SSR and capillary electrophoresis. A total of 229 DNA polymorphic bands Bands, 19 pairs of primers amplified DNA bands concentrated in the range of 100 ~ 260bp. Jaccard genetic similarity of 12 sugarcane materials, the minimum 0.09, the maximum 0.65, an average of 0.26. According to the analysis of genetic similarity coefficient, 12 sugarcane materials in UPGMA cluster can be divided into two groups. Three materials of cuttage germplasm are divided into one subgroup, and sugarcane cultivars and sugarcane tropical species are a subgroup. The results showed that the tropical species had more genetic relationship with sugarcane cultivars than the other species. The combination of SSR molecular markers with capillary technique was more accurate, simple and automatic than other molecular markers or electrophoresis techniques.