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目的了解当地莱姆病的主要生物媒介—蜱的种类、带菌状况以及在传播莱姆病中的作用。方法采用布旗法及动物诱捕法在山林地区采集蜱,对蜱种进行鉴定。并进行病原分离培养和PCR检测。结果共收集到1226只蜱,经鉴定属于硬蜱科中2属2种:硬蜱属的全沟硬蜱和血蜱属的长角血蜱。其中以长角血蜱为优势种,构成比占92.58%(1135/1226);随机对300只长角血蜱进行针对莱姆病螺旋体的PCR检测,有14只阳性,阳性率为4.67%。并对其余926只蜱进行病原分离培养,未得到莱姆病螺旋体。结论血蜱可能是天津蓟县地区莱姆病传播的主要生物媒介。
Objectives To understand the major biological mediators of local Lyme disease - the species of ticks, the prevalence of infection, and the role of transmission in Lyme disease. Methods The cloth flag method and the animal trapping method were used to collect ticks in the mountain forest area to identify the ticks. Pathogen isolation and culture and PCR detection. Results A total of 1226 ticks were collected and identified as two genera and two species of Ixodidae: Ixodes Ixodes and Haemophilus psittaci. Among them, P. longicornis was the dominant species, accounting for 92.58% (1135/1226). A total of 14 samples were positive for PCR detection of 300 Borrelia species with a positive rate of 4.67%. The remaining 926 ticks were isolated and cultured, and no Borrelia burgdorferi was obtained. Conclusion The blood-borne ticks may be the main bio-media of Lyme disease transmission in Ji County, Tianjin.