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研究植物蛇床Cnidium monnieri干燥成熟果实中的香豆素类成分及其细胞毒活性,为进一步开发利用蛇床子提供依据。采用大孔树脂、硅胶和Sephadex LH-20、C18柱色谱等方法分离纯化,并运用波谱方法对所分离的化合物进行结构鉴定。采用MTT法对所分离得到的化合物进行L1210癌细胞毒活性实验。从蛇床子中分离鉴定了11个香豆素类化合物,分别为蛇床子素(1)、佛手柑内酯(2)、花椒毒酚(3)、花椒毒素(4)、欧前胡素(5)、异虎耳草素(6)、欧前胡素酚(7)、补骨脂素(8)、5,7-二甲氧基香豆素(9)、水合羟基前胡内酯(10)、swietenocoumarin F(11)。化合物7,9~11为首次从蛇床属植物中分离得到。在样品溶液浓度为1×10-5mol·L-1的条件下,化合物1,5,10,11对小鼠白血病L1210细胞体外生长的抑制率分别是70.13%,63.10%,55.77%,75.08%,表明上述化合物对L1210细胞具有较明显的体外细胞毒活性。
The coumarins in Cnidium monnieri dry ripe fruit and its cytotoxic activity were studied in order to provide basis for further development and utilization of Cnidium monnieri. Macroporous resin, silica gel and Sephadex LH-20, C18 column chromatography and other methods were isolated and purified, and the use of spectral methods to identify the structure of the compounds isolated. The compounds obtained by MTT method were tested for L1210 cancer cell cytotoxic activity. Eleven coumarin compounds were isolated and identified from Cnidium monnieri (Osthol), and were identified as Osthole (1), Bergamot lactone (2), Zygote (3), Schisandra (4), Imperatorin 5), isooxanide (6), imperatorin (7), psoralen (8), 5,7-dimethoxycoumarin (9), hydrahydroxyprogesterone (10), swietenocoumarin F (11). Compounds 7 and 9 ~ 11 were isolated from Cnidium for the first time. The inhibitory rates of compound 1, 5, 10 and 11 on the in vitro growth of mouse leukemia L1210 cells were 70.13%, 63.10%, 55.77% and 75.08% at the concentration of 1 × 10-5mol·L-1, respectively , Indicating that the above compound has obvious cytotoxic activity on L1210 cells in vitro.