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目的:研究血府逐瘀汤含药血清对血管内皮细胞血凝素样氧化低密度脂蛋白受体-1(LOX-1)、肿瘤坏死因子-α(TNF-α)、血管细胞黏附因子-1(VCAM-1)及细胞间黏附分子-1(ICAM-1)表达的影响,探讨血府逐瘀汤防治动脉粥样硬化的机制。方法:血管内皮细胞随机分为对照组、模型组、西药对照组和血府逐瘀汤组,用LPS刺激2 h后分别加入阿托伐他汀和血府逐瘀汤含药血清干预,24 h后收集细胞,用荧光定量PCR方法和Western blot方法测定LOX-1、TNF-α、VCAM-1及ICAM-1的基因及蛋白表达。结果:用LPS刺激内皮细胞后,引起LOX-1、TNF-α、VCAM-1及ICAM-1的表达升高,差异有统计学意义(与对照组比较P<0.01),而用血府逐瘀汤含药血清干预以后显著抑制LOX-1、TNF-α、VCAM-1及ICAM-1的表达,差异有统计学意义(与模型组比较P<0.05)。结论:血府逐瘀汤可抑制LOX-1、TNF-α、VCAM-1及ICAM-1等炎症因子的表达,这可能是其防治动脉粥样硬化作用的机制之一。
Objective: To investigate the effects of Xuefu Zhuyu Decoction-containing serum on the expression of LOX-1, TNF-α, vascular cell adhesion molecule- 1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression in rats and explore the mechanism of Xuefuzhuyu Decoction in preventing and treating atherosclerosis. Methods: The vascular endothelial cells were randomly divided into control group, model group, western medicine control group and Xuefu Zhuyu Decoction group. After stimulated by LPS for 2 hours, they were given atorvastatin and Xuefuzhuyu Decoction serum separately. After 24 h The cells were collected and the gene and protein expressions of LOX-1, TNF-α, VCAM-1 and ICAM-1 were determined by fluorescence quantitative PCR and Western blot. Results: After stimulated with LPS, the expression of LOX-1, TNF-α, VCAM-1 and ICAM-1 were increased, the difference was statistically significant (P <0.01 compared with the control group) Compared with model group, P <0.05). The serum levels of LOX-1, TNF-α, VCAM-1 and ICAM-1 were significantly inhibited after treated with stasis-containing serum. Conclusion: Xuefu Zhuyu Decoction can inhibit the expression of inflammatory factors such as LOX-1, TNF-α, VCAM-1 and ICAM-1, which may be one of the mechanisms of prevention and treatment of atherosclerosis.