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采用静水呼吸法研究了中华鳑(1.070±0.068)g在溶氧逐渐降低环境中呼吸耗氧和氮代谢生理水平的变化。结果显示温度在(23.2±0.2)℃时,中华鳑饱食和饥饿状态下耗氧率分别为0.441 mg/(g·h)和0.297 mg/(g·h),排氨率分别为1.6μg/(g·h)和0.5μg/(g·h);溶氧低于1.5 mg/L开始浮头,低于0.31 mg/L开始死亡。当溶氧降低时耗氧率和排氨率均显著降低,但仍表现出一定的昼夜节律性,白天(6:00~14:00时)较夜间(10:00~2:00)高。在低溶氧环境中氨氮、亚硝酸氮浓度逐渐增加,水质环境随时间延长会逐渐恶化。实验结果提示在溶氧逐渐降低的环境胁迫下中华鳑的呼吸作用和氮代谢水平会逐渐下降。因此,在养殖和运输中华鳑过程中溶氧水平需始终保持在1.5 mg/L以上,运输前停食进行饥饿运输较佳。
The changes of respiratory oxygen demand and nitrogen metabolism in the declining dissolved oxygen environment of C. sinensis (1.070 ± 0.068) g were studied by using the water-respiration method. The results showed that under the conditions of (23.2 ± 0.2) ℃, the rates of oxygen consumption in the state of satiety and starvation were 0.441 mg / (g · h) and 0.297 mg / (g · h) respectively, and the rates of ammonia excretion were 1.6 μg / (g · h) and 0.5 μg / (g · h) respectively. Dissolved oxygen below 1.5 mg / L started floating head and began to die below 0.31 mg / L. Oxygen consumption rate and ammonia excretion rate decreased significantly when dissolved oxygen decreased, but it still showed some circadian rhythms. It was higher during daytime (6: 00-14: 00) than nighttime (10: 00-2: 00). In low dissolved oxygen environment ammonia nitrogen, nitrite nitrogen concentration gradually increased, the water quality environment will gradually deteriorate over time. The experimental results suggest that the respiration rate and nitrogen metabolism level of A. chinensis will gradually decrease under the environmental stress of gradually decreasing dissolved oxygen. Therefore, the dissolved oxygen level should always be above 1.5 mg / L during the culture and transportation of Chinese shrews. It is better to leave for food before starvation for starvation.