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从籼稻品种中籼3037第1染色体短臂、第4染色体长臂及第11染色体长臂端三体的自交后代中,选择形态性状出现明显变异但体细胞染色体数目仍为2n=24的个体.用水稻着丝粒特异的BAC克隆17p22为探针对有丝分裂早中期染色体进行荧光原位杂交(FISH)分析,各筛选到了一单端体植株,其体细胞中各含有一条端着丝粒染色体.进一步分别以位于第1染色体短臂、第4染色体长臂及第11染色体长臂上的特异分子细胞学标记a0059H02,a0034E24及a0071H11为探针,对以上3个单端体的有丝分裂早中期染色体及减数分裂粗线期的染色体进行荧光原位杂交分析,证明这些变异株确为第1染色体短臂、第4染色体长臂和第11染色体长臂的单端体.这些单端体的植株矮小,结实率较低.
From the indica rice indica 3037 first chromosome short arm, chromosome 4 long arm and chromosome 11 long arm end trisomic self-progeny, morphological traits obvious variation but the number of somatic chromosomes is still 2n = 24 individuals The rice centromere-specific BAC clone 17p22 was used as a probe to probe the mitotic metaphase chromosomes by FISH, and each single-terminal plant was screened. The somatic cells contained one telocentric chromosome Respectively.Moreover, the molecular markers a0059H02, a0034E24 and a0071H11, located on the short arm of chromosome 1, the long arm of chromosome 4 and the long arm of chromosome 11, were further used to probe the mitotic metaphase chromosome And meiosis pachytene chromosomes were analyzed by fluorescence in situ hybridization, which proved that these mutants were indeed the single arm of the short arm of chromosome 1, the long arm of chromosome 4 and the long arm of chromosome 11. These single-ended plants Short, low seed rate.