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目的研究1,1’-(联苯基-4,4’)-二[3-(二甲氨基)-1-丙酮](BDBDP)对东莨菪碱致小鼠阿尔茨海默病(AD)的作用及其机制。方法将50只ICR小鼠随机分为正常组、模型组及BDBDP低、中、高剂量组。连续ip给药18 d,第13天ip东莨菪碱造模,并采用Morris水迷宫及Y迷宫进行行为学测试;测定小鼠脑组织中乙酰胆碱酯酶(ACh E)、胆碱乙酰转移酶(Ch AT)、超氧化物歧化酶(SOD)的活性。结果 BDBDP能缩短水迷宫实验中小鼠的逃避潜伏期,并提高目标象的限滞留时间;可提高Y迷宫试验中小鼠的自发交替率,且不影响小鼠的活动能力;还能降低小鼠脑组织中ACh E的活性,提高Ch AT和SOD的活性。结论 BDBDP对东莨菪碱所致小鼠AD模型有明显改善作用,其机制可能与其作用于胆碱能系统及其抗氧化作用有关。
AIM To investigate the effects of 1,1 ’- (biphenyl-4,4’) - bis [3- (dimethylamino) -1-propanone] (BDBDP) on scopolamine-induced Alzheimer’s disease And its mechanism. Methods 50 ICR mice were randomly divided into normal group, model group and BDBDP low, medium and high dose groups. The mice were injected ip for 18 days, scopolamine ip on the 13th day, behavioral tests were performed using the Morris water maze and the Y maze. The ACh E and ChAT (acetylcholinesterase) ), Superoxide dismutase (SOD) activity. Results BDBDP can shorten the escape latency of mice in water maze test and increase the retention time of target mice. It can increase the spontaneous alternation rate of mice in Y maze test without affecting the activity of mice. It can also decrease the brain tissue of mice In ACh E activity, improve Ch AT and SOD activity. Conclusion BDBDP can significantly improve the AD model of mice induced by scopolamine, and its mechanism may be related to its role in the cholinergic system and its anti-oxidative effects.