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利用RFLP技术,比较研究了在农业生产上广泛应用的9种细胞质雄性不育体系的线粒体基因组,结果表明:1)9种水稻雄性不育细胞质的遗传相似性变化范围为 0.615～1.000。所有配子体雄性不育细胞质的遗传相似性变化范围为0.643～1.000,其中 QXA, DY1A 和 YM15A 这 3 种细胞质的遗传相似性为 1.000。所有孢子体雄性不育细胞质的遗传相似性为1.000;2)在配子体细胞质雄性不育水稻中,用3个探针(atpA, atp9+nad6, cox2)与2种内切酶(HindⅢ,BamHⅠ)的组合未发现不育系与保持系之间的多态性, 但在探针 atp6, cob, 和 nad2 的杂交带型中找到了不育系和保持系之间的一些差异。YTA和YTB 在5个探针-内切酶组合 (atp6/HindⅢ,cob/HindⅢ,atp6/BamHⅠ,cob/BamHⅠ,nad2/BamHⅠ)中存在差异。3种细胞质 (QX,SJ,DY1)的不育系和保持系之间的差异是相同的, 都出现在atp6/HindⅢ,atp6/BamHⅠ,cob/BamHⅠ等3个组合中。YM15A和YM15B 在4个组合(atp6/HindⅢ,atp6/BamHⅠ,cob/BamHⅠ,nad2/BamHⅠ)中存在差异。LYA 和 LYB 的差异出现在 cob/HindⅢ,cob/BamHⅠ,nad2/BamHⅠ这 3 个组合中;3)在孢子体细胞质雄性不育水稻中, 所有不育系的带型是一样的,所有保持系的带型也一样。不育系和保持系的差异出现在 atp6/HindⅢ,cob/HindⅢ,atp6/EcoRⅠ,cob/EcoRⅠ,cox1/EcoRⅠ,atp6/BamHⅠ,cob/BamHⅠ,cox1/BamHⅠ,cox2/BamHⅠ等 9 个组合中。这些结果在分子水平上揭示了9种雄性不育细胞质的线粒体基因组存在结构多样性,并为其细胞质雄性不育分子机理的研究打下了基础。
The mitochondrial genomes of 9 cytoplasmic male sterility systems widely used in agricultural production were compared using RFLP technology. The results showed that: 1) The genetic similarity of 9 cytoplasmic male sterile cytoplasms varied from 0.615 to 1.000. The genetic similarity of all the gametophytic male sterile cytoplasms ranged from 0.643 to 1.000, of which the genetic similarity of QXA, DY1A and YM15A was 1.000. The genetic similarity of all the sporophyte male sterile cytoplasms was 1.000; 2) Three probes (atpA, atp9 + nad6, cox2) and two endonucleases (HindⅢ, BamHⅠ) were used in gametophytic cytoplasmic male sterile rice Did not find polymorphisms between CMS and maintainer lines, but some differences between CMS and maintainer lines were found in the hybridization patterns of probes atp6, cob, and nad2. YTA and YTB differed in 5 probe-endonuclease combinations (atp6 / HindIII, cob / HindIII, atp6 / BamHI, cob / BamHI, nad2 / BamHI). The differences between the three cytoplasmic (QX, SJ, DY1) CMS lines and maintainer lines were the same and all appeared in three combinations of atp6 / HindⅢ, atp6 / BamHⅠ, cob / BamHⅠ. YM15A and YM15B differed in 4 combinations (atp6 / HindIII, atp6 / BamHI, cob / BamHI, nad2 / BamHI). The differences of LYA and LYB appeared in the three combinations of cob / HindⅢ, cob / BamHⅠand nad2 / BamHⅠ. 3) In the sporophytic male sterile rice, the genotypes of all CMS lines were the same, and all the maintainer lines The same type of band. Differences in CMS lines and maintainer lines occurred in 9 combinations of atp6 / HindIII, cob / HindIII, atp6 / EcoRI, cob / EcoRI, cox1 / EcoRI, atp6 / BamHI, cob / BamHI, coxl / BamHI, cox2 / BamHI . These results revealed at molecular level the structural diversity of the mitochondrial genomes of the nine male-sterile cytoplasm and laid the foundation for its molecular mechanism of cytoplasmic male sterility.