用二乙基亚硝胺 (DEN)诱发大鼠肝癌动物模型 ,以青椒碱治疗 ,分离肝细胞原代培养 ,应用整装细胞电镜技术结合选择性抽提观察细胞骨架。结果显示DEN诱发大鼠肝癌细胞的骨架丝状结构遭到破坏、断裂并聚集成团块 ,治疗组细胞骨架有明显的恢复。提示青椒碱可通过影响细胞骨架对大鼠实验性肝癌细胞起到治疗作用 Rat liver cancer model induced by diethylnitrosamine (DEN) was treated with green pepper alkali, and primary hepatocyte culture was separated. Cell cytoskeleton was observed by selective cell extraction combined with whole cell electron microscopy. The results showed that the skeletal filamentous structure of hepatoma cells in rats induced by DEN was destroyed, broken and aggregated into clumps, and the cytoskeleton in the treatment group was significantly restored. Tips that pepper base can be used to treat experimental hepatoma cells in rats by influencing cytoskeleton