目的：观察逆转录病毒载体介导的降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）基因对人脐静脉内皮细胞的作用，探讨其应用于动脉粥样硬化和经皮腔内冠状动脉成形术后再狭窄基因治疗的可行性。方法：构建含有ＣＧＲＰｃＤＮＡ的逆转录病毒载体，体外转染人脐静脉内皮细胞。用ＲＴＰＣＲ及放射免疫法分析检测基因表达水平。应用细胞计数及流式细胞仪观察ＣＧＲＰ基因对内皮细胞生长的影响。结果：逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。导入ＣＧＲＰ基因可以促进内皮细胞的分裂增殖，从而加速损伤内皮的修复。结论：ＣＧＲＰ基因有可能作为动脉粥样硬化及再狭窄基因治疗的有效候选基因 OBJECTIVE: To observe the effect of retroviral vector-mediated calcitonin gene related peptide (CGRP) on human umbilical vein endothelial cells and to explore its application in atherosclerosis and percutaneous transluminal coronary angioplasty After restenosis gene therapy feasibility. Methods: The retroviral vector containing CGRP cDNA was constructed and transfected into human umbilical vein endothelial cells in vitro. RT  PCR and radioimmunoassay to detect gene expression levels. The effect of CGRP gene on endothelial cell growth was observed by cell counting and flow cytometry. Results: The retroviral vector effectively introduced exogenous gene into vascular endothelial cells and stably expressed. The introduction of CGRP gene can promote endothelial cell division and proliferation, thus accelerating the damage of endothelial repair. Conclusion: CGRP gene may be a useful candidate gene for gene therapy of atherosclerosis and restenosis