报道经硫酸鱼精蛋白沉淀、硫酸铵分级沉淀和葡聚糖凝胶G-200柱层析,再经冰冻干燥后从温特曲霉F-871菌体中获得延胡索酸酶,纯化倍数为31.70,回收率为36.64％,酶比活性为24.6U/mg。酶学性质研究表明:酶作用最适pH和温度分别为8.0和30℃,稳定pH范围为6.0～8.5,酶在35℃下保温30min后仍残留约90％以上的活力。 Reported by protamine sulfate precipitation, ammonium sulfate fractionation and Sephadex G-200 column chromatography, and then freeze-dried fumarate from Aspergillus Wotherm F-871 obtained furarase, the purification fold was 31.70, recovery The rate was 36.64%, and the specific enzyme activity was 24.6U / mg. The results of enzymology showed that the optimum pH and temperature of enzyme activity were 8.0 and 30 ℃, respectively, and the stable pH range was 6.0 ~ 8.5. The enzyme still retained about 90% activity after 30min incubation at 35 ℃.