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已往资料中已有很多关于测定血清胆固醇含量方法的报导,一般多采用Zlatkis等人的三氯化铁-冰乙酸-硫酸试剂为显色剂。此反应较Lieberman与Burchard的反应稳定灵敏,且方法简单准确,故很多作者,如Mann,Leffler等人,Tu等人在测定血清与食物中胆固醇含量时皆用此显色剂。但Zlatkis等人的FeCl_3-HAC-H_2SO_4试剂在反应中释放出HCl,易产生气泡和影响反应温度,并且由于FeCl_3在空气中易潮解,给操作带来一定的困难,因此,Par-
In the past data have been reported on the determination of serum cholesterol content, the general use of Zlatkis et al. Ferric chloride - acetic acid - sulfuric acid reagent as the developer. This reaction is more stable and sensitive than Lieberman and Burchard. Many authors, such as Mann, Leffler et al. And Tu et al., Used this reagent in the determination of cholesterol content in serum and food. But Zlatkis et al.’s FeCl 3 -HAC-H 2 SO 4 reagent releases HCl in the reaction, easy to produce bubbles and affect the reaction temperature, and due to the deliquescence of FeCl 3 in the air, it brings some difficulties to the operation. Therefore, Par-