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孢子分离法是食用菌制种的常用方法之一,在教学、科研和生产中广为应用,并成为选育良种的重要途径。目前各地流行的孢子分离法形式多样,下面介绍我们所用的方法:1.孢子采集器消毒在培养皿内铺放数张长3—5厘米、宽0.8—1厘米的滤纸条,装上铁丝折成的支架,再将培养皿放入瓷盘,盖上玻璃罩,用双层纱布包好,置于每平方厘米1.1公斤的高压锅中消毒0.5小时,移入接种室备用。
Spore separation is one of the common methods for seed production of edible fungi. It is widely used in teaching, research and production and has become an important way to breed elite seeds. At present, the prevalence of spore separation method in various forms, the following describes the method we used: 1. Spore collector disinfection in the Petri dishes placed a few 3-5 cm long, 0.8-1 cm wide filter paper, fitted with wire Folded into the stent, and then the dish into the porcelain dish, cover glass, wrapped with double gauze, placed 1.1 kg per square centimeter autoclave sterilized 0.5 hours, into the vaccination room.