论文部分内容阅读
目的 观察NO能否改善链脲佐菌素 (STZ)糖尿病大鼠高糖毒性导致的胰岛素敏感性下降。方法 对清醒状态下的STZ糖尿病大鼠(24只)和正常大鼠 (24只 )在实施正血糖高胰岛素钳夹实验同时,分别给予硝普钠、NG 甲基 L 精氨酸(LNMMA)及腺苷,观察葡萄糖代谢率 (MCR,作为胰岛素作用的指标)的变化。结果 糖尿病大鼠生理盐水组的MCR〔(7. 2±0. 8)ml·kg-1·min-1〕明显低于正常对照大鼠〔(18. 0±1. 8 )ml·kg-1·min-1,P<0. 01〕,给予LNMMA的糖尿病大鼠的MCR〔( 5. 0±0. 2 )ml·kg-1·min-1〕有进一步下降趋势,但是给予硝普钠的糖尿病大鼠的MCR〔(15. 3±1. 5)ml·kg-1·min-1,P<0. 01〕明显高于糖尿病大鼠生理盐水组,达到了正常大鼠MCR的 85%,而腺苷没有明显改变糖尿病大鼠的MCR〔(7. 1±0. 7)ml·kg-1·min-1〕。结论 NO可以改善STZ糖尿病大鼠高糖毒性导致的胰岛素作用下降。
Objective To observe whether NO can improve the insulin sensitivity induced by hyperglycemia in streptozotocin (STZ) diabetic rats. Methods Simultaneous normoglycemic and hyperinsulinemic clamp experiments were performed in awake STZ diabetic rats (24 rats) and normal rats (n = 24). Simultaneously, sodium nitroprusside, NG methyl L arginine (LNMMA) Adenosine was observed for changes in glucose metabolic rate (MCR, as an indicator of insulin action). Results Compared with normal control rats, MCR 〔(7.2 ± 0.8) ml · kg-1 · min-1〕 in diabetic rats was significantly lower than that in normal control rats 〔(18.0 ± 1.8) ml · kg- 1 · min-1, P <0.01), MCR 〔(5. 0 ± 0. 2) ml · kg-1 · min-1〕 in diabetic rats given LNMMA showed a further downward trend, MCR 〔(15.3 ± 1.5) ml · kg-1 · min-1, P <0.01) in diabetic rats was significantly higher than that in diabetic rats and reached the level of MCR in normal rats 85%, while adenosine did not significantly change the MCR in diabetic rats 〔(7.1 ± 0.7) ml · kg -1 · min -1]. Conclusion NO can improve the insulin action induced by hyperglycemia in STZ diabetic rats.