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目的探讨乌鲁木齐地区维、汉族儿童支气管哮喘与ADAM33基因多态性的相关性。方法选取同期3~15岁于乌鲁木齐地区生活的86例维族和111例汉族哮喘患儿,同时纳入64例汉族、56例维族健康儿童为对照组;采用聚合酶链反应对ADAM33基因V4、T2位点进行单核苷酸多态性(SNP)分型,并对其中部分进行基因检测验证。结果哮喘组和对照组之间,ADAM33基因V4、T2位点三种基因型的分布差异有统计学意义(P均<0.01);V4位点哮喘组CC基因型频率较高,与G等位基因个体比较,C等位基因携带者儿童发生哮喘的风险增加1.51倍(95%CI:1.10~2.09);T 2位点哮喘组AA基因型频率较高,与G等位基因个体比较,A等位基因携带者儿童发生哮喘的风险增加1.96倍(95%CI:1.32~2.91)。汉族儿童中,哮喘组和对照组之间ADAM 33基因V 4、T 2位点三种基因型分布的差异无统计学意义(P均>0.05);而维族儿童中,V 4、T 2位点三种基因型分布的差异有统计学意义(P均<0.05)。结论 ADAM 33基因V 4、T 2位点与乌鲁木齐地区维族儿童哮喘发病相关。
Objective To investigate the relationship between polymorphism of ADAM33 gene and bronchial asthma in Victoria and Han nationality children in Urumqi. Methods Eighty-six Uygur and 111 Han children with asthma were enrolled in this study. Sixty-six Han and 56 Uygur children were enrolled in this study. Polymerase chain reaction (PCR) was used to detect the expression of ADAM33 gene V4 and T2 Single point nucleotide polymorphism (SNP) typing, and some of them for genetic testing. Results There were significant differences in the distribution of ADAM33 gene V4 and T2 genotypes between the asthma group and the control group (P <0.01). The frequency of CC genotype in the V4 site asthma group was higher than that in the G allele Individuals with C alleles had a 1.51-fold increased risk of asthma (95% CI: 1.10 to 2.09) in children with the C allele; AA genotypes were higher in the T 2 locus compared with those with the G allele Children with alleles had a 1.96-fold increased risk of developing asthma (95% CI: 1.32-2.91). Among Han children, there was no significant difference in the genotype distributions of VAM4 and T 2 between the asthma group and the control group (P> 0.05). Among the Uygur children, V 4 and T 2 The differences of the distribution of the three genotypes were statistically significant (P <0.05). Conclusion The V 4 and T 2 loci of ADAM 33 gene are associated with asthma in Uighur children in Urumqi.