论文部分内容阅读
目的探讨应用荧光原位杂交(FISH)技术检测宫颈脱落细胞中TERC基因的最佳方法。方法采集门诊就诊77例患者的宫颈脱落细胞,分别以生理盐水直接制片、生理盐水低渗制片和LCT低渗制片,应用FISH技术检测宫颈脱落细胞中TERC基因,分析比较检测效果。结果生理盐水直接制片、生理盐水低渗制片和LCT低渗制片的TERC基因杂交成功率分别是62.5%、82%、79%,差异无统计学意义(P>0.05);背景清晰比例分别是52.4%、81%、78.5%,裸核细胞比例分别是31.2%、68.7%、69.7%,差异有统计学意义(P<0.05)。结论应用荧光原位杂交技术检测宫颈脱落细胞TERC基因时,生理盐水低渗制片和LCT低渗制片背景清晰,杂交信号强,杂交成功率高,且生理盐水低渗制片杂交效果最满意。
Objective To investigate the best method of detecting TERC gene in exfoliated cells by fluorescence in situ hybridization (FISH). Methods The exfoliated cells were collected from 77 outpatients in clinic. The normal cervical epithelial cells were directly injected with physiological saline, hypobaric saline and LCT respectively. FISH was used to detect TERC gene in cervical exfoliated cells, and the results were analyzed and compared. Results The success rates of TERC gene hybridization between saline direct preparation, saline hypobaric preparation and LCT hypotonic preparation were 62.5%, 82% and 79%, respectively, with no significant difference (P> 0.05) (52.4%, 81%, 78.5%, respectively), and the percentage of nudeal cells were 31.2%, 68.7% and 69.7%, respectively. The difference was statistically significant (P <0.05). Conclusions When detecting the TERC gene in cervical exfoliated cells by fluorescence in situ hybridization, there is a clear background in the hypotonic saline and LCT hypotonic slices with strong hybridization signal and high success rate of hybridization, and the most satisfactory results are achieved in the hybridization with physiological saline hypotonic membrane .