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探讨益气、活血、温阳、利水4种治法治疗心力衰竭的机制及配伍规律。方法:将益气、活血、温阳、利水四大法作为4个影响因素,分别选取代表药物,以高、中、低剂量作为3个水平,按L9(34)正交表配伍组方,煎液灌胃,另设空白对照组。观察各含药血清对去甲肾上腺素、氯化钾、维拉帕米、酚妥拉明预作用的体外兔主动脉条收缩张力的影响。结果:各中药组含药血清对去甲肾上腺素和氯化钾、去甲肾上腺素和维拉帕米引起的体外兔主动脉条收缩有较明显的抑制作用,与空白血清相比有显著性差异(P<0.05)。各中药组含药血清对去甲肾上腺素和酚妥拉明引起的体外兔主动脉条收缩无明显的抑制作用,与空白血清相比无显著性差异(P>0.05)。依据含药血清对去甲肾上腺素和氯化钾引起的体外兔主动脉条收缩的抑制率结果所作的方差分析显示,各因素和结果相关性的强弱顺序为温阳、利水、益气、活血。结论:温阳、利水、益气、活血法的不同配伍均有扩张血管作用,该作用是通过阻滞α受体实现的,与钙通道的关系不大。最优配伍方案为温阳药高剂量、利水药和益气药均中剂量、活血药低剂量。
To explore the mechanism and compatibility of four kinds of therapies for the treatment of heart failure by Qi-Qi, Huoxue, Wenyang and Lishui. Methods: The four methods of Qi-invigorating, blood-activating, warming-yang, and diuresis were taken as four influencing factors. Drugs were selected to represent the drug. High, medium, and low doses were used as the three levels. The L9 (34) orthogonal table was used to formulate the formula. Liquid gavage, another blank control group. Observe the effect of each drug-containing serum on the contraction tension of noradrenaline, potassium chloride, verapamil, and phentolamine in vitro of rabbit aortic strips. Results: The drug-containing serum of each Chinese medicine group had obvious inhibitory effect on norepinephrine and potassium chloride, norepinephrine, and verapamil induced contraction of rabbit aortic strips in vitro, which was significant compared with blank serum. Difference (P<0.05). The drug-containing serum of each Chinese medicine group had no obvious inhibitory effect on the contraction of rabbit aortic strips induced by norepinephrine and phentolamine, and there was no significant difference compared with blank serum (P>0.05). An analysis of variance based on the inhibition rate of noradrenaline and potassium chloride caused by noradrenaline and potassium chloride in vitro rabbit aortic strip contraction showed that the order of strength of each factor and result was Wenyang, Lishui, Qi, Blood circulation. Conclusion: Different combinations of Wenyang, Lishui, Qi and Huoxue all have the effect of dilating blood vessels. This effect is achieved by blocking α-receptors, and has little to do with calcium channels. The optimal compatibility program was high doses of Wenyang medicine, Lishui medicine and Qi-invigorating medicine were all medium dose, and Huoxue medicine was low dose.