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作者观察了人工合成的反义c-Ha-ras和 c-myc寡聚脱氧核昔酸(ASO-r,ASO-m)对两株胃癌细胞中Ha-ras癌基因表达和细胞生长增殖的影响。观察到ASO-r能显著抑制MGc-803细胞P21蛋白合成(作用12 h抑制率达48.10％,P<0.01),同时对其DNA合成和细胞增殖也有较显著的抑制作用(抑制率分别为76.79％,55.61％,P<0.05)。ASO-r对SGc-7901细胞的DNA合成和细胞增殖也有类似的抑制作用(抑制率分别为76.78％,62.02％,P<0.05)。ASO-m对两株细胞的细胞增殖和SGc-7901细胞DNA合成均无明显影响,仅对MGc-803细胞的DNA合成有抑制作用(抑制率为71.37％,P<0.05)。结果表明:ASO-r能够阻断c-Ha-ras癌基因表达,并抑制胃癌细胞生长增殖;c-Ha-ras癌基因可能是维持胃癌细胞恶性生长表型的主要因素。
The authors observed the effects of synthetic antisense c-Ha-ras and c-myc oligodeoxynucleotide (ASO-r, ASO-m) on Ha-ras oncogene expression and cell growth and proliferation in two gastric cancer cells. . It was observed that ASO-r could significantly inhibit the synthesis of P21 protein in MGc-803 cells (the inhibition rate was 48.10% at 12 h, P<0.01). At the same time, it also had significant inhibitory effect on DNA synthesis and cell proliferation (76.79, respectively). %, 55.61%, P<0.05). ASO-r also had similar inhibition on DNA synthesis and cell proliferation of SGc-7901 cells (the inhibition rates were 76.78% and 62.02%, respectively, P<0.05). ASO-m had no significant effect on cell proliferation and DNA synthesis of SGc-7901 cells in both cells and only inhibited DNA synthesis of MGc-803 cells (inhibition rate was 71.37%, P<0.05). The results showed that ASO-r can block the expression of c-Ha-ras oncogene and inhibit the growth and proliferation of gastric cancer cells. c-Ha-ras oncogene may be the main factor to maintain the malignant growth phenotype of gastric cancer cells.