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本文根据甘兰型油菜细胞核雄性不育性是由二对显性基因上位互作控制的假设,通过试验,把核不育系内二种稳定型不育两型系(简称两型系)——纯合两型系和杂合两型系鉴别出来,然后用纯合两型系的不育株与双隐性可育株测交,获得100％不育的全不育系,用它与恢复系制种。这样,核不育系的利用由原来的二系(两型系与恢复系)制种法,变为三系(纯合两型系、双隐性保持系、恢复系)制种法。三系制种法比二系制种法可节省用工量,且能提高制种效率,为核不育系的杂种优势利用开拓了一条更切实可行,又经济有效的制种途径。
Based on the hypothesis that the dominant male sterility in Brassica napus was controlled by the interaction of two dominant genes, the two types of sterile two-type genotypes (two-type strains) - homozygous two-type and heterozygous two-type lines were identified, and then homozygous two-type sterile lines and double recessive fertile lines measured cross, access to 100% sterile all-male sterile line, with it and Restore the system seed. In this way, the use of nuclear sterile lines from the original two lines (two-type and restore lines) seed production method, into three lines (homozygous two-type, double recessive maintenance, restorement) seed production method. Three-line seed production method than the second-line seed production method can save labor, and can improve the efficiency of seed production, heterosis utilization of nuclear sterile lines to open up a more practical and cost-effective seed production approach.