日本血吸虫成虫23kDa蛋白(Sj23)是一种整合膜蛋白,针对Sj23蛋白的单克隆抗体I-134,具有免疫诊断的潜在价值。本文报告了已从cDNA基因库中鉴定、克隆出Sj23蛋白,对其进行了进一步的研究。按已报道的方法,利用载体λZAP和λgt11构建日本血吸虫(Sj)cDNA表达基因库,已克隆并提纯的、用作杂交探针的Sm23或Sj23插入子采用随机引物法进行标记。以~(32)P标记的Sm23插入子在Sjλgt11cDNA The adult schistosoma japonicum 23 kDa protein (Sj23) is an integrated membrane protein that is potentially valuable for the immunodiagnosis of the monoclonal antibody I-134 against the Sj23 protein. This paper reports that Sj23 protein has been identified and cloned from cDNA gene library and further studied. According to the reported method, the cDNA library of Schistosoma japonicum (Sj) cDNA was constructed by using vectors λZAP and λgt11. The cloned and purified Sm23 or Sj23 insert, which was used as hybridization probe, was labeled with random primers. Sm23 insert labeled with ~ (32) P in Sjλgt11 cDNA