通过建构扩增模型和酶切模型,可以突破基因工程中的PCR和酶切方法这两个难点。在此过程中应加强对学生的思维训练,使其深入感知科学本质和微观生命,从中领悟和形成运用模型建构方法的能力,提高思维强度,延展思维宽度,提升思维深度。 By constructing the amplification model and the enzyme digestion model, we can overcome the two difficulties of PCR and enzyme digestion in genetic engineering. In this process, we should strengthen the training of students’ thinking so that they can deeply perceive the nature of science and microscopic life, understand and form the ability to use the model to construct methods, enhance the thinking intensity, extend the width of thinking, and enhance the thinking depth.