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目的:研究麻杏石甘汤剂中盐酸麻黄碱和苦杏仁苷的含量测定方法。方法:采用C_(18)反向色谱柱,流动相:乙腈-甲醇-0.1%磷酸(含0.04%三乙胺,0.02%正丁胺)(1:1:8),柱温:30℃,检测波长:210nm。结果:盐酸麻黄碱和苦杏仁苷进样量分别在0.175-1.050μg,0.461-2.766μg范围内峰面积呈良好线性关系。回收率分别为:盐酸麻黄碱99.26%,RSD=2.42%;苦杏仁苷99.30%,RSD=1.67%。一剂方中含盐酸麻黄碱13.47mg/g(n=3),含苦杏仁苷44.30mg/g(n=3)。结论:本方法操作简单、快速、准确、灵敏度高、专属性强、重现性好,可作为麻杏石甘汤剂的定量控制方法之一。
Objective: To study the content determination of ephedrine hydrochloride and amygdalin in Maxing Shigan Decoction. METHODS: C_ (18) reversed-phase column was used. The mobile phase was acetonitrile-methanol -0.1% phosphoric acid (0.04% triethylamine and 0.02% n-butylamine) Detection wavelength: 210nm. Results: The peak areas of ephedrine hydrochloride and amygdalin in the range of 0.175-1.050μg and 0.461-2.766μg, respectively, showed a good linear relationship. The recoveries were as follows: ephedrine hydrochloride 99.26%, RSD = 2.42%; amygdalin 99.30%, RSD = 1.67%. One dose contains ephedrine hydrochloride 13.47mg / g (n = 3), with amygdalos 44.30mg / g (n = 3). Conclusion: The method is simple, rapid, accurate, sensitive, specific and reproducible. It can be used as a quantitative control method for Maxing Shigan Decoction.