论文部分内容阅读
利用反相HPLC分析对ECG和EGCG的氧化产物的色谱进行了比较。实验中有少量的EGCG在所设计的氧化体系中,由ECG经所选择的化学氧化条件氧化形成。所以ECG的氧化可能是先产生EGCG,随后再与EGCG共同被氧化或EGCG单独被氧化来继续其反应。用于分析洗脱红茶及多酚类的氧化产物的较为理想的HPLC洗脱梯度应为:试剂A1%柠檬酸,自92%至50%,而相应的试剂B乙腈,则由8%在设计的区间内升至50%。根据对高聚合氧化产物的重点分析,在这种洗脱梯度下仅用15分钟便可以全部洗脱出叫做茶灰素的组分。相关研究表明多酚类的氧化反应即使在很低的温度下亦会自发地进行。
The chromatograms of the oxidation products of ECG and EGCG were compared using reverse phase HPLC analysis. In the experiment, a small amount of EGCG was formed in the designed oxidation system by the oxidation of ECG by the selected chemical oxidation conditions. Therefore, the oxidation of ECG may first produce EGCG, followed by oxidation with EGCG or EGCG oxidation alone to continue its reaction. The preferred HPLC elution gradient used to analyze the oxidation products that elute black tea and polyphenols should be: Reagents A1% Citric Acid, from 92% to 50%, while the corresponding Reagents, B Acetonitrile, from 8% The interval rose to 50%. Based on a key analysis of the polymerized oxidation products, the components called tealin eluted completely in just 15 minutes at this elution gradient. Related research shows that the oxidation of polyphenols even spontaneously at very low temperatures.