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在弱酸性缓冲溶液中,乙基曙红(EE)、赤藓红(ET)和荧光桃红(PX)3种卤代荧光素与盐酸西布曲明(SH)形成离子缔合物,导致吸收光谱发生变化和荧光猝灭。研究了反应产物的吸收和荧光光谱特征,适宜的反应条件,据此建立了以卤代荧光素为光谱探针的灵敏、简便、快速测定SH的新方法。其中ET体系褪色反应灵敏度最高,对SH的线性范围为0.1～4.0μg/mL,检出限为0.06μg/mL;PX-SH体系的荧光猝灭法对SH的线性范围是0.2～4.6μg/mL(λex/λem=540nm/560nm),检出限为0.09μg/mL。讨论了离子缔合反应对荧光及吸收光谱的影响及卤代荧光素荧光猝灭和褪色的原因。
In weakly acidic buffer solution, three kinds of halo-fluorescein, such as ethyl eosin (EE), erythrosine (ET) and fluorescent pink (PX), formed ionic complexes with sibutramine hydrochloride (SH) Spectral changes and fluorescence quenching. The absorption and fluorescence characteristics of the reaction products and the suitable reaction conditions were studied. A new method for sensitive, simple and rapid determination of SH using halo-fluorescein as a spectral probe was established. Among them, the fading reaction sensitivity of ET system was the highest, the linear range of SH was 0.1-4.0μg / mL and the limit of detection was 0.06μg / mL. The linear range of fluorescence quenching method for PX-SH system was 0.2-4.6μg / mL (λex / λem = 540nm / 560nm), the detection limit was 0.09μg / mL. The effect of ionic association reaction on fluorescence and absorption spectra and the reasons of fluorescence quenching and fading of halo-fluorescein were discussed.