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利用高效液相色谱法检测了中豆27(♀)×九农20(♂)重组自交系的异黄酮含量。结果显示重组自交系间异黄酮含量差异显著。分析了异黄酮含量同主要农艺性状和化学品质性状的相关性,结果表明:总异黄酮含量与蛋白含量、脂肪含量、蛋白脂肪总含量、分枝数、单株产量、总荚数、主茎荚数和分枝荚数呈现负相关,其中与蛋白含量和蛋白脂肪总含量相关都分别达到显著和极显著水平;同株高、主茎节数和百粒重呈正相关趋势,并进一步估算了各异黄酮的含量与主要农艺性状的通径系数。
The content of isoflavones in Zhongnong 27 (♀) × Jiunong 20 (♂) recombinant inbred lines was detected by high performance liquid chromatography. The results showed that the content of isoflavones in recombinant inbred lines was significantly different. The results showed that the contents of total isoflavones, protein content, fat content, total protein and fat content, number of branches, single plant yield, total pods, main stem There was a negative correlation between the number of pods and the number of pods. The correlation between the number of pods and the number of pods was significant and extremely significant, respectively. Path coefficient of each isoflavone content to major agronomic traits.