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目的:建立宣肺止咳合剂中葛根素含量的HPLC测定方法。方法:采用Hypersil BDS C18分析柱(250mm×4.6 mm,5μm),流动相为甲醇∶水=25∶75,流速为1.0 mL·min-1,检测波长为250 nm,柱温为30℃。结果:本方法线性范围为7.0625~226μg/mL(r=0.9999),平均回收率在100.58%,RSD为2.31%(n=6)。结论:本方法灵敏准确,重复性好,适用于宣肺止咳合剂中葛根素的含量测定。
Objective: To establish HPLC method for determination of puerarin in Xuanfei Zhike Mixture. METHODS: Hypersil BDS C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol: water = 25:75, the flow rate was 1.0 mL · min-1, the detection wavelength was 250 nm and the column temperature was 30 ℃. Results: The linear range of this method was 7.0625 ~ 226μg / mL (r = 0.9999). The average recovery was 100.58%. The RSD was 2.31% (n = 6). Conclusion: The method is sensitive and accurate, good repeatability, suitable for determination of puerarin in Xuanfei Zhike Mixture.