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A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized.The indicator exhibits a highly selective ratiometric emission response to Ca~(2+) over other metal cations and a large Stokes shift of 202 nm.Moreover,its practical cell imaging capability for intracellular Ca~(2+) in the resting- and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.
A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized. The indicator exhibits a highly selective ratiometric emission response to Ca ~ (2+) over other metal cations and a large Stokes shift of 202 nm. Moreover, its practical cell imaging capability for intracellular Ca ~ (2+) in the resting- and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.