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利用光镜、扫描电镜和透射电镜技术对栉孔扇贝(Chlamysferreri)血细胞与细胞免疫功能相关的几个因素进行了初步研究。对血细胞的数量和不同功能细胞的比例研究结果表明,健康血淋巴中血细胞的平均密度为3.03±0.11×107cell/ml,其中颗粒细胞占42.6%,透明细胞占57.4%;病贝血淋巴中血细胞的平均密度为2.78±0.34×107cell/ml,其中颗粒细胞占40.2%,透明细胞占59.8%。扫描电镜观察表明,血细胞的表面结构主要有表面光滑型,表面松果型和表面褶皱阿米巴型3类。透射电镜观察表明,颗粒细胞吞噬外源性颗粒(Ⅰ型颗粒)通过溶酶体(Ⅱ型颗粒)进行降解。并观察到同心片层结构出现在吞噬泡的降解过程中。利用APIZYM试剂盒对栉孔扇贝血细胞及血清中的19种酶进行检测,结果在血清中检测到了13种酶,在血细胞中检测到10种酶,健康血淋巴中酶的含量高于病贝。对血细胞吞噬活性的研究结果表明,血细胞对大肠杆菌和对类立克次体(RLO)的吞噬率分别为25.4%和21.7%。颗粒细胞的吞噬活性(30%-40%)远远大于透明细胞(4.8%-14%)。环境胁迫对血细胞吞噬活性的影响的研究结果表明,病原菌感染和温度、盐度等环境胁迫因素对血细胞的吞噬活性均有不同程度的影响,其中高温因素影响较大,但未发现贝龄有显著影响
Several factors related to cellular immune function of Chlamys farreri blood cells were studied by light microscopy, scanning electron microscopy and transmission electron microscopy. The results showed that the average density of blood cells in healthy hemolymph was 3.03 ± 0.11 × 107cell / ml, of which 42.6% were granulocytes and 57.4% were clear cells; the blood cells in hemolymph The average density of 2.78 ± 0.34 × 107cell / ml, of which 40.2% of granulosa cells, transparent cells accounted for 59.8%. Scanning electron microscopy showed that the surface structure of blood cells are mainly smooth surface, surface pine cones and surface fold amoeba type 3. Transmission electron microscopy showed that exocytosis of granulosa cells (type I granules) was degraded by lysosomes (type II granules). The concentric lamellar structure was observed during the degradation of phagocytic vesicles. Thirteen kinds of enzymes were detected in serum by using APIZYM kit for 19 kinds of enzymes in blood and serum of Chlamys farreri, 10 kinds of enzymes were detected in serum, and the content of enzymes in healthy hemolymph was higher than that in Siberian shellfish. The results of hemophagocytic activity showed that the phagocytosis rate of blood cells to Escherichia coli and RLO was 25.4% and 21.7%, respectively. Granulocyte phagocytosis activity (30% -40%) is much larger than that of clear cells (4.8% -14%). Environmental stress on the hemophagocytic activity of the results showed that pathogenic bacteria infection and temperature, salinity and other environmental stress factors on the phagocytic activity of blood cells have varying degrees of impact, including high temperature factors, but did not find a significant effect influences