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目的探讨环磷酸腺苷反应元件结合蛋白(CREB)在儿童急性淋巴细胞白血病(ALL)中的表达及意义。方法1.实时定量反转录-聚合酶链反应(RT-PCR)法检测ALL患儿骨髓单个核细胞CREB的mRNA表达量,以非白血病(NL)患儿10例作对照,对9例ALL患儿进行动态测定。2.ELISA法测定ALL患儿骨髓单个核细胞CREB总蛋白和蛋白磷酸化水平表达。结果1.高危组(HR)CREB的mRNA表达量明显高于NL组、低危组(LR)、中危组(MR),分别为8.49±12.24、1.13±0.81、1.47±1.02、1.24±0.60,Pa<0.05;而其他各组无明显差异。2.HR组CREB总蛋白量(ng/106个细胞)明显高于LR、MR组,分别为0.99±0.43、4.40±4.60,Pa<0.05;HR组CREB蛋白磷酸化的量(U/106个细胞)明显高于LR、MR组,分别为1.95±1.82、8.67±8.19,Pa<0.05。3.MICM分型与CREB高表达相结合,HR-ALL患儿的诊断符合率可达85.0%。4.CREB的mRNA表达量升高提示ALL未缓解或复发。结论CREB在儿童HR-ALL中呈高表达。CREB的表达量及其动态监测对ALL患儿的临床分型和疗效判断有一定的意义。
Objective To investigate the expression and significance of cyclic adenosine monophosphate response element binding protein (CREB) in childhood acute lymphoblastic leukemia (ALL). Method 1. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of CREB in ALL patients. Ten children with non-leukemia (NL) Children with dynamic determination. The expression of CREB protein and protein phosphorylation of bone marrow mononuclear cells in ALL patients were detected by ELISA. The mRNA expression of CREB in high risk group (HR) was significantly higher than that in NL group, low risk group (LR) and middle risk group (MR), respectively, 8.49 ± 12.24,1.13 ± 0.81,1.47 ± 1.02,1.24 ± 0.60 , Pa <0.05; while no significant difference in other groups. The total CREB protein content (ng / 106 cells) in HR group was significantly higher than those in LR and MR groups (0.99 ± 0.43,4.40 ± 4.60, Pa <0.05). The CREB protein phosphorylation Cells were significantly higher than those in LR and MR groups (1.95 ± 1.82 and 8.67 ± 8.19, respectively, P <0.05) .MICM classification combined with high expression of CREB had a diagnostic coincidence rate of 85.0% in children with HR-ALL. 4.CREB mRNA expression increased prompted ALL did not relieve or relapse. Conclusion CREB is highly expressed in children HR-ALL. The expression of CREB and its dynamic monitoring of ALL children with clinical classification and curative effect have a certain significance.