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为了解各型病毒性肝炎抗HCV情况,我们于1991年4月～1991年12月对我院住院的223例病毒性肝炎病人血清进行抗HCV、抗HAVIGM、HBVM及HB-VDNA检测,现将检测结果及临床研究报告如下.1 资料和方法1.1 病例选择 按1990年第六届全国病毒性肝炎会议(上海)所制定的标准诊断和分型.223例病毒性肝炎中甲肝50例,慢乙肝152例(其中乙型慢迁肝73例,乙型慢活肝76例,乙型肝炎后肝硬化3例),非甲非乙型肝炎21例.男144例,女79例.年龄3～71岁,平均34岁.1.2 检测方法 抗HCV检测采用ELISA法,使用美国ABBOTT公司抗HCV检测药盒(第二代),操作方法按说明书进行.抗HAVIGM及HBVM采用ELISA法检测,试剂由中国军事医学科学院提供.HB-VDNA采用生物素法,试剂由北京医科大学肝病研究所提供.
To understand the anti-HCV status of various types of viral hepatitis, we tested the anti-HCV, anti-HAVIGM, HBVM and HB-VDNA sera of 223 patients with viral hepatitis hospitalized in our hospital from April 1991 to December 1991, Test results and clinical studies are as follows.1 Materials and Methods 1.1 Case Selection According to the standard of the sixth National Conference of Hepatitis (Shanghai) in 1990 diagnosis and classification of 223 cases of viral hepatitis in 50 cases of hepatitis A, chronic hepatitis B 152 cases (including 73 cases of slow-moving liver B, 76 cases of slow-acting hepatitis B, 3 cases of post-hepatitis B cirrhosis), 21 cases of non-A, non-B hepatitis, including 144 males and 79 females, 71 years old, average 34 years old.1.2 Detection method Anti-HCV test using ELISA method, the use of the United States ABBOTT company anti-HCV test kit (second generation), the method according to the instructions.HAVIGM and HBVM anti-HAVIGM by ELISA, reagents from China Military Academy of Medical Sciences .HB-VDNA biotin method, reagent provided by the Beijing Medical University Liver Institute.