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目的检测某水源水及经常规处理后的管网末梢水的遗传毒性,了解氯化消毒前后水质遗传毒性变化。方法将水样经XAD-2树脂柱富集其中的有机物,正己烷和丙酮的混合液(1:1)洗脱,旋转蒸发仪浓集后分别用玉米油、二甲基亚砜定容,冰箱贮存备用。利用成组生物试验(Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠肝细胞凝胶电泳试验)从不同的遗传学终点检测其遗传毒性。结果水源水只在单细胞凝胶电泳试验最高剂量时出现阳性结果,其他两项试验为阴性结果,末梢水以上三项试验结果与阴性对照有显著差异,均为阳性结果。结论在本实验剂量范围内经过氯化消毒处理后的管网末梢水有遗传毒性。
Objective To detect the genotoxicity of some water sources and the conventional pipe network water after treatment, and to understand the changes of genotoxicity before and after chlorination. Methods The water samples were concentrated by XAD-2 resin column and eluted with a mixture of n-hexane and acetone (1: 1). After concentration by rotary evaporator, the samples were fixed with corn oil and dimethylsulfoxide (DMSO) Refrigerator storage spare. Genotoxicity was tested at various genetic endpoints using a panel bioassay (Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test, mouse hepatocyte gel electrophoresis assay). Results The water source water showed positive results only at the highest dose of single cell gel electrophoresis test. The other two tests were negative results. The results of the three tests above the peripheral water were significantly different from the negative control and were positive results. Conclusion In the experimental dose range after chlorination disinfection of the pipe end of the water genotoxicity.