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目的:研究类黄酮化合物槲皮素(quercetin,QUE)对体外培养的大鼠脑胶质瘤C6细胞增殖调控的作用。方法:按QUE浓度分成10、25、50、75及100μmol.L-15个处理组和空白对照组(生理盐水)及溶剂对照组(二甲亚砜),大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106.mL-1后,在96孔板中分别加入上述浓度的QUE继续培养,每组设3复孔,作用24、48及72 h,采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况,流式细胞术(FCM)对50及100μmol.L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析,免疫组化法检测50μmol.L-1的QUE作用48 h的p53和bcl-2基因产物。结果:与空白对照组比较,QUE处理组随药物浓度增加和作用时间的延长,A值减小(P<0.05),对C6细胞的增殖抑制作用增强,使停滞在G0/G1期的细胞增加(P<0.01),而S和G2/M期细胞减少(P<0.05)。P53蛋白表达增加和Bcl-2蛋白表达减少。结论:QUE对C6细胞增殖抑制作用具有浓度、时间依赖性,通过P53蛋白表达增加和Bcl-2蛋白表达减少诱导细胞凋亡来实现。
AIM: To investigate the effect of quercetin (QUE), a flavonoid compound, on the proliferation of C6 glioma cells cultured in vitro. Methods: The QUE concentrations were divided into 10, 25, 50, 75 and 100μmol.L-15 treatment groups and blank control group (saline) and solvent control group (dimethyl sulfoxide) After the growth of RPMI 1640 medium reached 1 × 106 mL-1, the above QUEs were added to 96-well plates and cultured for 3, 4, 48 and 72 h in each group. MTT colorimetry The inhibitory effect of QUE on C6 glioma cells in vitro was analyzed by flow cytometry (FCM). The effects of QUE on 50 and 100 μmol·L-1 QUE were evaluated on the C6 glioma cells of 48 h after 48 h. Histochemistry was used to detect the effect of QUE at 50μmol.L-1 for 48 hrs on p53 and bcl-2 gene products. Results: Compared with the blank control group, the QUE treatment group decreased the A value (P <0.05) with the increase of the drug concentration and the prolongation of the action time, and enhanced the proliferation of the C6 cells and increased the cells arrested in the G0 / G1 phase (P <0.01), while cells in S and G2 / M phase decreased (P <0.05). P53 protein expression increased and Bcl-2 protein expression decreased. CONCLUSION: QUE inhibits the proliferation of C6 cells in a concentration-dependent and time-dependent manner, which is achieved through the increase of P53 protein expression and the decrease of Bcl-2 protein expression.