寄生虫疫苗的制备和寄生虫免疫诊断,均需大量纯净的抗原。作者利用体细胞杂交瘤及体外培养技术,将肝片吸虫成虫细胞与BALB/C小鼠Sp2株骨髓瘤细胞融合,在HAT培养基中克隆、亚克隆,融合后分泌寄生虫抗原达1年。材料及方法:寄生虫和骨髓瘤细胞的融合、筛选和生长:肝片吸虫成虫用含抗生素的PBS洗涤数次,再剪成细小的碎片置于含 Preparation of parasite vaccine and parasite immune diagnosis, require a large number of pure antigens. Using somatic hybridomas and in vitro culture techniques, adult cells of Fasciola hepatica were fused with Sp2 myeloma cells of BALB / C mice and cloned, subcloned and fused in HAT medium for 1 year. Materials and Methods: Fusion, Screening and Growth of Parasite and Myeloma Cells: Adult Fasciola hepatica were washed several times with antibiotic-containing PBS and then cut into small pieces