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以黄瓜绿斑驳病毒(Cucumber green mottle mosaic virus)野生株系为试材,采用高温处理、亚硝酸处理及复合处理对其进行人工诱变,比较3种诱变方法致弱效果。结果表明:3种方法均具有削弱病毒群体在苋色藜上枯斑扩展的能力,野生株系接种苋色藜后,直径小于1mm枯斑比率为1.99%,亚硝酸处理、高温处理、复合处理小枯斑比率分别为11.41%、12.87%、14.20%,复合处理致弱效果最好。该结果有助于快速有效的筛选CGMMV弱毒株,也将为弱毒疫苗的开发和致病相关基因功能的研究奠定基础。
The cucumber green mottle mosaic virus was used to mutagenize the wild strain of Cucumber green mottle mosaic virus by high temperature treatment, nitrite treatment and composite treatment. Weak effects of three mutagenesis methods were compared. The results showed that all the three methods had the ability of attenuating the spread of brown spot on the population of amaranth, and the percentage of dead spots with the diameter of less than 1mm was 1.99% when wild strain was inoculated with amaranth, while nitrite, high temperature and compound treatment The ratio of small blight was 11.41%, 12.87% and 14.20% respectively, and the composite treatment had the weakest effect. The results will help to screen the CGMMV attenuated strains rapidly and effectively, and lay a foundation for the development of attenuated vaccine and the study of the function of disease-related genes.