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从拟南芥中克隆热激蛋白基因HSP18.2的启动子区域,构建了由HSP18.2启动子引导的GUS融合基因,并经农杆菌介导导入籼稻愈伤组织中。对热激和常温条件下转基因籼稻愈伤组织中GUS活性的比较测定表明,HSP18.2启动子在热激条件下可驱动GUS报告基因在转基因愈伤组织中高效表达,而常温条件下GUS活性在检测水平以下,证明HSP18.2启动子在籼稻中对基因的调控十分灵敏。
The promoter region of heat shock protein gene HSP18.2 was cloned from Arabidopsis thaliana, and the GUS fusion gene which was induced by HSP18.2 promoter was constructed and introduced into indica rice callus via Agrobacterium tumefaciens. Comparison of the GUS activity of transgenic callus in heat shock and normal temperature showed that the HSP18.2 promoter could drive the GUS reporter gene to be highly expressed in transgenic callus under the condition of heat shock and the GUS activity Below the detection level, it was demonstrated that the HSP18.2 promoter is very sensitive to gene regulation in indica rice.