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巢式ＰＣＲ方法被应用于泰国疟区（Ｂｏｒａｉ）恶性疟原虫的基因分型。应用特异的等位基因引物扩增了恶性疟原虫裂殖子表面蛋白１（ＭＳＰ１）基因的１、４变异多态区。以凝胶电泳分析扩增的目的基因片段。结果表明：共分别检测出恶性疟原虫６个ＭＡＤ２０等位基因型、４个Ｋ１和１个ＲＯ３３等位基因型。在９个月的流行季节，上述虫株的等位基因频率没有明显的改变，关存在较高程度的恶性疟原虫不同等位基因株的混合感染。本文进一步阐明了巢式ＰＣＲ技术在疟疾流行病学上的应用前景及优点。
The nested PCR method was applied to genotyping Plasmodium falciparum in Borai, Thailand. The alleles of Plasmodium falciparum MSP1 1 and 4 polymorphisms were amplified by using specific allele primers. The amplified target gene fragment was analyzed by gel electrophoresis. The results showed that six MAD20 alleles, four K1 alleles and one RO33 allele were detected in P. falciparum. At the 9-month epidemic season, there was no significant change in the allele frequencies of the above strains, suggesting a higher level of mixed infection with different alleles of Plasmodium falciparum. This article further elucidates the prospects and advantages of nested PCR in the application of malaria epidemiology.