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通过室内试验与温室试验研究了具有生防能力的木霉菌株TrichodermaMM35所分泌的胞外水解酶在拮抗大豆根腐病病原菌(F.oxysporum、R.solani)中的作用。试验结果表明:以病原菌F.oxysporum烘干的菌丝体作唯一碳源,可以诱导TrichodermaMM35分泌几丁质酶、β-1,3-葡聚糖酶。β-1,3-葡聚糖酶高水平诱导表达在前,几丁质酶诱导表达在后。土壤中接种TrichodermaMM35、F.oxysporum和R.so-lani之后都能够检测到几丁质酶、β-1,3-葡聚糖酶活性。向有病原菌F.oxysporum的土壤中接种Tri-chodermaMM35,土壤中几丁质酶活性能够显著升高。向有病原菌R.solani的土壤中接种TrichodermaMM35,土壤中的几丁质酶、β-1,3-葡聚糖酶活性都显著升高。
The effects of extracellular hydrolase secreted by TrichodermaMM35, a biocontrol agent against Trichoderma spp., On the pathogen of soybean root rot (F.oxysporum, R. solani) were studied by laboratory and greenhouse experiments. The results showed that Trichoderma MM35 could induce the secretion of chitinase and β-1,3-glucanase from the mycelium of F.oxysporum which was dried by pathogen Fusarium oxysporum. High levels of β-1, 3-glucanase induce the expression of chitinase induced expression in the former. Chitinase and β-1,3-glucanase activities were detected in soil after inoculation of TrichodermaMM35, F.oxysporum and R. so-lani. Tri-chodermaMM35 was inoculated into soil with pathogen F. oxysporum, and the activity of chitinase in soil could be significantly increased. TrichodermaMM35 was inoculated into the soil of pathogen R. solani, the activities of chitinase and β-1,3-glucanase in soil were significantly increased.