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为研究亚砷酸钠(NaAsO2)的肝细胞毒性和氧化应激作用。NaAsO2(5、10、20、40、80、100和200μmol/L)染毒24 h,用Alamar Blue法检则细胞活力;NaAsO2(2.5、5、10和25μmol/L)染毒24 h,或NaAsO2(10μmol/L)染毒2 h、6 h、12 h和24 h,用流式细胞术检测细胞内2′,7′-二乙酰二氯荧光素(DCFH-DA)的荧光强度,间接反应细胞内氧化应激水平。结果NaAsO2(5~200μmol/L)染毒24 h能够显著降低Chang肝细胞的细胞活力,且具有剂量-效应关系(P<0.01);不同剂量NaAsO2(2.5、5和10μmol/L)染毒24 h,细胞内荧光强度明显增高,分别是对照组的1.67、1.96和2.30倍(P<0.01);而浓度10μmol/L的NaAsO2染毒组,细胞内荧光强度在12 h和24 h均显著高于对照组(P<0.01)。提示无机砷能够产生肝细胞毒性,增强细胞内的氧化应激水平,并且具有剂量和时间反应关系。
To study hepatotoxicity and oxidative stress of sodium arsenite (NaAsO2). Cells were exposed to NaAsO2 (5, 10, 20, 40, 80, 100 and 200 μmol / L for 24 h) The fluorescence intensity of intracellular 2 ’, 7’-diacetyl dichlorofluorescein (DCFH-DA) was detected by flow cytometry after exposed to NaAsO2 (10μmol / L) for 2 h, 6 h, 12 h and 24 h. Response to intracellular oxidative stress. Results NaAsO2 (5 ~ 200μmol / L) for 24 h could significantly reduce the cell viability of Chang hepatocytes with a dose-effect relationship (P <0.01). NaAsO2 (2.5, 5 and 10 μmol / L) h, the intracellular fluorescence intensity was significantly increased, which was 1.67,1.96 and 2.30 times higher than that of the control group (P <0.01). However, the fluorescence intensity of NaAsO2 exposed to 10μmol / L of NaAsO2 was significantly higher at 12 and 24 h In the control group (P <0.01). Tip inorganic arsenic can produce hepatotoxicity, increase the level of oxidative stress in cells, and has a dose and time response.