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本文报道用一对特异的Ｙ＿（１．１），Ｙ＿（１．２）引物对经温度、湿度、ｐＨ、福尔马林、乙醇和土埋处理的牙髓ＤＮＡ及室温保存的陈旧牙髓ＤＮＡ进行扩增。结果发现男性牙齿出现特异的１５４ｂｐ扩增带，女性未出现带。男性４５颗牙经环境及理化因素处理，其中经土埋、水泡和ｐＨ２各处理１０周及火烧１０分钟的牙髓ＤＮＡ未见特异的１５４ｂｐ扩增带；经６６％湿度、ｐＨ１０各处理１０周以及火烧５分钟后的牙髓ＤＮＡ扩增带较弱，其余均有１５４ｂｐ的扩增带出现；６颗高分子量ＤＮＡ和２４颗高分子量伴部分降解的ＤＮＡ经扩增能获得比较满意的结果：１１颗牙齿完全降解的ＤＮＡ除１颗ＰＣＲ扩增结果未显带外，其余都显带；４颗牙齿电泳未检出ＤＮＡ，除１颗ＰＣＲ扩增结果显带外其余皆无带，说明只要降解的ＤＮＡ中仍含有待扩增的靶ＤＮＡ序列，就可用ＰＣＲ扩增作性别鉴定。
In this paper, a pair of specific Y_ (1.1) and Y_ (1.2) primers were used to detect the presence of dental pulp DNA and temperature-humidity, pH, formalin, DNA for amplification. The results found that male teeth appear specific 154bp amplification band, the female did not appear with the band. Male 45 teeth were treated by environmental and physico-chemical factors. There was no specific 154bp band in dental pulp DNA treated with soil burial, blisters and pH2 for 10 weeks and fire for 10 minutes. After treatment with 66% humidity and pH 10 for 10 weeks And the dental pulp DNA amplification band after weakened for 5 minutes was weaker, and the remaining 154 bp amplification bands appeared; 6 high-molecular-weight DNA and 24 high-molecular-weight partially degraded DNA could obtain satisfactory results by amplification: 11 teeth completely degraded DNA except one PCR amplification results were not banding, the rest are banding; 4 teeth electrophoresis DNA was not detected, except for a result of PCR amplification banding out the rest without the band, indicating that as long as Degraded DNA still contains the target DNA sequence to be amplified, and PCR amplification can be used for sex identification.